Lab Report- Nursing

Lab: Using the Microscope
Before starting your lab assignment: Please read/watch the below.
How to use a Microscope:
How to use a Microscope: BioNetwork
https://www.youtube.com/watch?v=ROsc-IrJJ6M
Common Microscope Mistakes
https://www.youtube.com/watch?v=eZX9U15F5Q8

Elodea Plant and How to Make a Wet Mount: Some great information about plant cells
https://www.youtube.com/watch?v=b6_SuhG_VPM

Microscopic Images of Cells Loyola University Microscope and Cellular Diversity web page: • To view images, scroll down
the webpage. • Click on each image to view a larger picture.
https://www.luc.edu/biology/111lab/3-microscopyandcellulardiversity/
Pond Life Video Gallery: Nikon
https://www.microscopyu.com/galleries/dic-phase-contrast/pond-life

Purpose: Purpose restated in your own words (1 sentence max).
Key Terms: Please define the following terms. Remember to restate the terms in your own words.
 Low to high rule- Defined in your own words (1 sentence max).
 Cover slip- Defined in your own words (1 sentence max).
 Objective lens- Defined in your own words (1 sentence max).
 Diaphragm- Defined in your own words (1 sentence max).
 Stage- Defined in your own words (1 sentence max).

Post Lab:
Questions about how to use the microscope
1. Name 4 general suggestions for focusing your microscope. When increasing in magnification, do you
increase or decrease the amount of light? When are the course and fine focus knobs used during focusing?
Response in your own words
2. Describe the procedures for using the oil immersion lens (100X objective lens). Response in your own words
3. The second microscope video showed several microscope mistakes. List the mistakes and how to correct
them. Response in your own words
Loyola University Microscope and Cellular Diversity web page
4. Compare microscopic images of a plant cell and an animal cell (Elodea Leaf and Human Epidermal Cells).
State your observations including the following: Responses in your own words

 Color of cells?
 Shape or structure of cells?
 Can internal cellular structures such as the cell nucleus, chloroplasts, vacuole be observed?
5. Examine the microscopic images of bacteria (middle row of images on the Loyola University webpage).
 Compare the relative size of bacterial cells to plant cell. A quick and easy comparison is to compare the
magnification of the bacterial and plant images. What observations can you make? Response in your
own words
 Compare the three microscopic images of bacteria? Record your observations regarding shape and
structure of cells, color of cells (cells were stained for microscopic imaging) and any other observations.
Response in your own words

Use the Pond life gallery resource to watch the movement of living protozoan. Fifteen different examples of
protozoans (single celled eukaryotes, representative species include Amoebas and Paramecium)
are shown towards the bottom of the webpage.
6. Pick two different protozoans and list the protozoan name and 2 observations regarding either cellular shape
and structure of a description of the movement, feeding or another activity. Response in your own words